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KMID : 0368420040470030244
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Journal of Plant Biology
2004 Volume.47 No. 3 p.244 ~ p.253
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A study of cotyledon senescence in cucumber (Cucumis sativus L.) based on expressed sequence tags and gene expression
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Kim Dae-Jae
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Abstract
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Cucumber cotyledons provide an excellent experimental system in which to investigate developmental changes in gene expression, from the early phase of heterotrophism through phototrophic growth to senescence. A cDNA library was prepared from the final stage of senescing cucumber cotyledons (<95% yellow) for studying the genes responsible for lipid mobilization during germination and senescence. This library had produced numerous senescence-associated clones in a previous study. Here, a total of 365 cDNA clones and their expression levels were examined via semi-quantitative RT-PCR. Up-regulation of expression was detected for several known and unknown genes. These results were used to investigate the possible functions for senescence-related genes during cotyledon development
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KEYWORD
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cotyledon, cucumber, EST, RT-PCR, senescence
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